Transfection Reagents
HORIZON DISCOVERY BIOSCIENCES LIMITED ACCELL HU GAPD POOL 50NMOL
NC1439685 ACCELL HU GAPD POOL 50NMOL
Expression Systems 293-ES cells, Suspension in ESF SFM, 20M total cells in 15mL conical tube
Expression Systems offers 293 cell lines adapted to serum-free suspension culture in ESF SFM. The availability of pre-adapted cells saves significant time and expense associated with the adaptation of cultures.
293-ES cells are derived from the human embryonic kidney HEK 293 cell line. 293-ES cells are adapted for suspension culture in ESF SFM and are available as a frozen vial or suspension culture.
Capable of producing high levels of AAV vectors
Capable of high levels of recombinant protein expression
Good growth in adherent or suspension culture Quality and performance testing
Organism: Homo sapiens, human
Tissue: Embryonic Kidney
Cell Type: Epithelial
Biosafety Level 2: Cells contain adenovirus. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Lonza Walkersville SE Cell Line 96-well Nucleofector Kit (960 RCT)
The SE Cell Line 96-well Nucleofector Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL SE Cell Line Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)10 x 96-well Nucleocuvette Plate (20 μL)
HERA BIOLABS Super piggyBacTM transposase expression vector, 25 ug, 1.5 ml vial, Research Use Only
Super piggyBacTM transposase. Sequence has been codon optimized
New England Biolabs, Inc. NEB Turbo Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
Chemically competent E. coli competent cells suitable for high efficiency transformation and rapid colony growth.
Bio Rad Laboratories TRANSFECTIN LIPID REAGENT
Lipid Reagent; Bio-Rad; TransFectin; High efficiency and gene activity; Transfection at 50 to 90% culture confluence; Simple three-step protocol; Affordable; Storage: 4 deg.C; 1.0mL; Quantity: 5
Eurofins Discoverx KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B), 2 vials x 0.5 (Adherent), 2 vials x 1.0 (Suspension). (97-0008)
The KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-adherent-c-97-0008.
Eurofins Discoverx KILR Retroparticles for Adherent Cells (Hygromycin B), 4 vials x 0.5. (97-0005)
The KILR Retroparticles for Adherent Cells (Hygromycin B) are MMLV particles designed to transduce adherent cells. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-adherent-cells-(hygrom-97-0005.
LAMDA BIOTECH INC Tranfection reagent: LipoFexin
LipoFexin is a lipid-based transfection reagent that forms complex with DNA or RNA, and transports the complex into cells. It works on a variety of adherent and suspension cell lines. It can be used for transfection of either DNA or RNA into eukaryotic cells in culture media with or without serum. LipoFexin has been compared to work at the similar way and efficiency as the Lipofectamine 2000 transfection reagent. LipoFexin has the following Special Features: • Superior transfection efficiency for a broad range of cell lines. • No requirement of removal of serum from culture medium. • No requirement for washing or changing of medium after transfection. • Low cytotoxicity.
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.